Electronic(1)
14629
Kate,Abhijeet S.
Kohl,Amber C.
Kerr,Russell G.
A Simple and Sensitive Apci-Lc-Ms Method for the Detection of the Antitumor Agent, Carmustine (Bcnu) in Rat Plasma
Journal of Liquid Chromatography & Related Technologies
J.Liq.Chromatogr.Rel.Technol.
2010
04
33
6
818
824
ANTINEOPLASTIC agents
VINYL chloride
NITROSOUREAS
RATS as laboratory animals
LABORATORY animals
ANIMAL models in research
ATMOSPHERIC pressure
CHEMICAL ionization mass spectrometry
APCI
BCNU
carmustine
LC-MS
post column formate adduct
A simple and sensitive LC-MS method for the quantitative determination of the antitumor agent, N,N'-bis (2-chloroethyl)-N-nitrosourea (BCNU or carmustine), is described in this report. The method has been developed using an atmospheric pressure chemical ionization (APCI) probe on an ion trap mass spectrometer. A formate adduct ([M + 45]-) of BCNU was observed in the negative mode at 257.8 m/z and further confirmation for BCNU was obtained by observing the characteristic isotopic pattern in the mass spectrum, which indicated the compound contained two chlorine atoms. The extraction of BCNU from rat plasma with isopropyl ether:hexane (1:1) resulted in 81.3% of sample recovery. The limit of detection (LOD) calculated for the method was 1.25 ng (injected amount) using a standard solution of BCNU (S/N = 9:1). Optimum linearity and reproducibility data were obtained for BCNU; r2 = 0.9999 (0.2 µg/mL to 10.2 µg/mL) with precision of (RSD) ≤ 6.4% at two different concentrations and accuracy of 92.3 and 102.4%. [ABSTRACT FROM AUTHOR]; Copyright of Journal of Liquid Chromatography & Related Technologies is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
M3: Article
10826076
48795566
http://search.ebscohost.com/login.aspx?direct=true&db=aph&AN=48795566&site=ehost-live
10.1080/10826071003684331
rkerr
Chemistry
Unknown(0)